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human proximal renal tubular epithelial cell line  (ATCC)


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    ATCC human proximal renal tubular epithelial cell line
    Human Proximal Renal Tubular Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proximal+tubular+epithelial+cell+line+hk+2/HK-2/pm42262012-93-0-11
    Average 99 stars, based on 4555 article reviews
    human proximal renal tubular epithelial cell line - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Repurposing of FDA-approved drugs by targeting SIRT2 to alleviate inflammatory response and kidney injury.
    Article Snippet: .. The immortalized proximal tubular epithelial cell line HK-2 (CRL2190) isolated from the adult human kidney was obtained from the American Type Culture Collection (ATCC). .. The keratinocyte serum-free (KCSF) medium with 40 μg/mL bovine pituitary extract (Gibco BRL, Grand Island, NY, USA) and 5 ng/mL recombinant epidermal growth factor was used to maintain the HK-2 cells.

    Cell Culture:

    Article Title: Comprehensive profiling of cell type-specific expression and distribution of complement genes in mouse and human kidneys: insights into normal physiology and response to kidney transplantations
    Article Snippet: .. The human proximal tubular epithelial cell line HK-2 (ATCC, USA) was cultured in MEM medium supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin (Beyotime, China). .. Primary human mesangial cells (ScienCell, USA) were cultured in mesangial cell medium (ScienCell, USA) supplemented with mesangial cell growth supplement (ScienCell, USA) and 2% FBS (Gibco, USA).

    Article Title: Comprehensive profiling of cell type-specific expression and distribution of complement genes in mouse and human kidneys: insights into normal physiology and response to kidney transplantations
    Article Snippet: .. The human proximal tubular epithelial cell line HK-2 (ATCC, USA) was cultured in MEM medium supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillinstreptomycin (Beyotime, China). .. Primary human mesangial cells (ScienCell, USA) were cultured in mesangial cell medium (ScienCell, USA) supplemented with mesangial cell growth supplement (ScienCell, USA) and 2% FBS (Gibco, USA).

    Article Title: Elucidation of puberulic acid-induced nephrotoxicity using stem cell-based kidney organoids.
    Article Snippet: .. The immortalized human proximal tubular epithelial cell line HK-2 (American Type Culture Collection, USA) was cultured in DMEM/F12 supplemented with 10% FBS, were seeded in 96-well plates and incubated overnight at a density of 5,000 cells per well in 100 μL of medium to adhere. .. Then, puberulic acid was added at various concentrations (0.4–50 μM) and incubated for 24 h. The cell viability following puberulic acid treatment was assessed using Cell counting kit-8, according to the manufacturer’s protocol (Dojindo Laboratories, Japan), as previously described24.

    Article Title: Pseudogene-derived long non-coding RNAs GSM3P1/Gstm2-ps1 exacerbate sepsis-associated acute kidney injury by suppressing their parent gene translation.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Elucidation of puberulic acid–induced nephrotoxicity using stem cell-based kidney organoids
    Article Snippet: .. The immortalized human proximal tubular epithelial cell line HK-2 (American Type Culture Collection, USA) was cultured in DMEM/F12 supplemented with 10% FBS, were seeded in 96-well plates and incubated overnight at a density of 5,000 cells per well in 100 μL of medium to adhere. .. Then, puberulic acid was added at various concentrations (0.4–50 μM) and incubated for 24 h. The cell viability following puberulic acid treatment was assessed using Cell counting kit-8, according to the manufacturer’s protocol (Dojindo Laboratories, Japan), as previously described .

    Incubation:

    Article Title: Elucidation of puberulic acid-induced nephrotoxicity using stem cell-based kidney organoids.
    Article Snippet: .. The immortalized human proximal tubular epithelial cell line HK-2 (American Type Culture Collection, USA) was cultured in DMEM/F12 supplemented with 10% FBS, were seeded in 96-well plates and incubated overnight at a density of 5,000 cells per well in 100 μL of medium to adhere. .. Then, puberulic acid was added at various concentrations (0.4–50 μM) and incubated for 24 h. The cell viability following puberulic acid treatment was assessed using Cell counting kit-8, according to the manufacturer’s protocol (Dojindo Laboratories, Japan), as previously described24.

    Article Title: Elucidation of puberulic acid–induced nephrotoxicity using stem cell-based kidney organoids
    Article Snippet: .. The immortalized human proximal tubular epithelial cell line HK-2 (American Type Culture Collection, USA) was cultured in DMEM/F12 supplemented with 10% FBS, were seeded in 96-well plates and incubated overnight at a density of 5,000 cells per well in 100 μL of medium to adhere. .. Then, puberulic acid was added at various concentrations (0.4–50 μM) and incubated for 24 h. The cell viability following puberulic acid treatment was assessed using Cell counting kit-8, according to the manufacturer’s protocol (Dojindo Laboratories, Japan), as previously described .

    Western Blot:

    Article Title: Pseudogene-derived long non-coding RNAs GSM3P1/Gstm2-ps1 exacerbate sepsis-associated acute kidney injury by suppressing their parent gene translation.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    GSTM3P1/Gstm2-ps1 are transiently induced in kidney proximal tubular cells in sepsis-associated acute kidney injury models. BUMPT and HK-2 cells were treated with 100 μg/mL lipopolysaccharide (LPS) for 3, 6, and 12 hours. Male C57BL/6J mice were subjected to an i.p. injection of 10 mg/kg LPS or cecal ligation and puncture (CLP) surgery, and kidney samples were collected at 3, 6, and 9 hours after injury. A—D: Quantitative RT-PCR analysis of Gstm2-ps1 or GSTM3P1 expression. A: Gstm2-ps1 in BUMPT. B: GSTM3P1 in HK2. C: Gstm2-ps1 in LPS-treated mouse kidney. D: Gstm2-ps1 in mouse kidney with CLP. A—D: The statistical difference was determined using one-way analysis of variance with Tukey multiple comparison. E and F: Representative images of Gstm2-ps1 in kidney tissue assessed by in situ hybridization and costained with fluorescein isothiocyanate—labeled Lotus tetragonolobus lectin (LTL). E: Mouse kidneys with or without LPS treatment for 3 hours. F: Mouse kidneys with or without CLP injury for 3 hours. n 5 ( A—D ); n 6 ( E and F ). ** P < 0.01, *** P < 0.001. Scale bar 100 μm ( E and F ). NS, nonsignificant.

    Journal: The American journal of pathology

    Article Title: Pseudogene-Derived Long Noncoding RNAs GSTM3P1/Gstm2-ps1 Exacerbate Sepsis-Associated Acute Kidney Injury by Suppressing Their Parent Gene Translation

    doi: 10.1016/j.ajpath.2026.01.004

    Figure Lengend Snippet: GSTM3P1/Gstm2-ps1 are transiently induced in kidney proximal tubular cells in sepsis-associated acute kidney injury models. BUMPT and HK-2 cells were treated with 100 μg/mL lipopolysaccharide (LPS) for 3, 6, and 12 hours. Male C57BL/6J mice were subjected to an i.p. injection of 10 mg/kg LPS or cecal ligation and puncture (CLP) surgery, and kidney samples were collected at 3, 6, and 9 hours after injury. A—D: Quantitative RT-PCR analysis of Gstm2-ps1 or GSTM3P1 expression. A: Gstm2-ps1 in BUMPT. B: GSTM3P1 in HK2. C: Gstm2-ps1 in LPS-treated mouse kidney. D: Gstm2-ps1 in mouse kidney with CLP. A—D: The statistical difference was determined using one-way analysis of variance with Tukey multiple comparison. E and F: Representative images of Gstm2-ps1 in kidney tissue assessed by in situ hybridization and costained with fluorescein isothiocyanate—labeled Lotus tetragonolobus lectin (LTL). E: Mouse kidneys with or without LPS treatment for 3 hours. F: Mouse kidneys with or without CLP injury for 3 hours. n 5 ( A—D ); n 6 ( E and F ). ** P < 0.01, *** P < 0.001. Scale bar 100 μm ( E and F ). NS, nonsignificant.

    Article Snippet: Human proximal tubular epithelial cell line HK-2 (ATCC, Manassas, VA), rat proximal tubular cell line RPTC (gifted from Dr. Ulrich Hopfer, Case Western Reserve University, Cleveland, OH), mouse proximal tubular cell line BUMPT (gifted from Drs. Wilfred Lieberthal and John Schwartz, Boston University School of Medicine, Boston, MA), and human embryonic kidney cell line HEK293 (ATCC) were maintained as described previously.

    Techniques: Injection, Ligation, Quantitative RT-PCR, Expressing, Comparison, In Situ Hybridization, Labeling